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Image Search Results
Journal: Blood Science
Article Title: DNAH2 facilitates the homologous recombination repair of Fanconi anemia pathway through modulating FANCD2 ubiquitination
doi: 10.1097/BS9.0000000000000076
Figure Lengend Snippet: DHAH2 knockdown impairs DNA HR repair of U2OS cells. (A) DR-U2OS cell line with the I-SceI expression vector were used for the HR assay. The schematic diagram of the I-SceI/GFP reporter system was displayed. (B) DR-U2OS cell line was transfected with the siControl, siDNAH2, or siFANCA, respectively. the GFP signal was detected by a flow cytometric analysis. (C) The proportion of positive cells with GFP signals was calculated, respectively. The ANOVA followed by the LSD test was then performed. ANOVA: analysis of variance; DNAH2 , Dynein Axonemal Heavy Chain 2; GFP: green fluorescent protein; HR: homologous recombination; LSD: least significant difference.
Article Snippet: Under the transfection of I-Scel expression plasmid (pCBASce), the GFP signal was detected by a
Techniques: Knockdown, Expressing, Plasmid Preparation, Transfection, Homologous Recombination